Other
Mod. Bact.

Part:BBa_K1787001:Design

Designed by: Joy R. L. Santos   Group: iGEM15_NAIT_Edmonton   (2015-09-18)


Modified Bacteria Lipoprotein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order for our sequence to be synthesized, we had to intercalate non-polar amino acids between the polar amino acids. This, in order to make the protein that is synthesized by the sequence more reactive with the silver staining reagents.


Source

The DNA sequence encoding for the protein was modified so as to reduce non-polar amino acids in between the polar amino-acids. It was introduced into BBa_K1321338 (containing a T7 promoter with a RBS).

References